Original Assignee Heng Ruiyuan Zheng Guangzhou Biotechnology Co ltd Hengrui Yuanzheng Shenzhen Biological Technology Co ltd Henry Is Source Of Biological Science And Technology Co ltd Shanghai Priority date (The priority date is an assumption and is not a legal conclusion. Henry Is Source Of Biological Science And Technology Co ltd Shanghai Hengrui Yuanzheng Shenzhen Biological Technology Co ltd Heng Ruiyuan Zheng Guangzhou Biotechnology Co ltd Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Inventor 石先灯 李进 吴健华 Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.) Pending Application number CN202010129401.2A Other languages Chinese ( zh)
#Capto plasmid select pdf#
Google Patents Method for large-scale rapid purification of plasmid DNAĭownload PDF Info Publication number CN111304193A CN111304193A CN202010129401.2A CN202010129401A CN111304193A CN 111304193 A CN111304193 A CN 111304193A CN 202010129401 A CN202010129401 A CN 202010129401A CN 111304193 A CN111304193 A CN 111304193A Authority CN China Prior art keywords plasmid dna concentration column purification anion exchange Prior art date Legal status (The legal status is an assumption and is not a legal conclusion. Google Patents CN111304193A - Method for large-scale rapid purification of plasmid DNA RNA endotoxin hydrophobic interaction ionic recognition multimodal chromatography plasmid DNA.CN111304193A - Method for large-scale rapid purification of plasmid DNA The chromatographic separation behavior was very robust between pH values 6 and 9, and the dynamic binding capacity was estimated to 60 µg/ml resin. The RNA fraction bound most strongly to the resin and could be eluted only at very high salt concentrations (2.0 M NaCl). Using a stepwise NaCl gradient, pure plasmid DNA could be obtained without protein and endotoxin contaminations. This property could be used to isolate plasmid DNA from a crude Escherichia coli extract.
Capto Adhere proved to offer a very strong binding of nucleic acids. Capto Adhere is a recent multimodal chromatography material allowing molecular recognition between the ligand and target molecule, which is based on combined ionic and aromatic interactions. The binding potential and chromatographic behavior of plasmid DNA have here been examined on a Capto Adhere resin. Multimodal chromatography is widely used for isolation of proteins because it often results in improved selectivity compared to conventional separation resins.
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